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United States Department of Agriculture

Agricultural Research Service

Title: Transmission of Maize Rough Dwarf Fijivurus by Vascular Puncture Inoculation with Leaf Extracts and Partially Purified Preparations from Root Extracts

item Louie, Raymond
item Gordon, Donald - OARDC-OSU
item Bradfute, Oscar - OARDC-OSU (RETIRED)

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 1995
Publication Date: N/A

Technical Abstract: Virus transmission from extracts of leaves of plants naturally infected with maize rough dwarf fijivirus (MRDV) was achieved by vascular puncture inoculation (VPI) of maize kernels. MRDV was purified from roots of these infected plants by clarifying extracts with carbon tetrachloride or Freon 113 or initially concentrating without clarification using polyethylene glycol. After a low speed centrifugation and then a high speed sucrose density gradient (30, 40, 50, and 60%) centrifugation, the banded virus was collected and dialyzed against the extraction buffer (0.05M Na2 HPO4, 0.005M sodium EDTA and 0.01M Na2SO3, pH 7.8). Best rates of transmission (100 kernels/test) by VPI from leaf extracts and partially purified preparations involving root extracts clarified with carbon tetrachloride or Freon 113 or initially concentrated with polyethylene glycol were 48, 47, 48, and 40%, respectively. Infected plants were stunted and exhibited veinal chlorosis, swollen veins, and enations. Fijivirus-like particles also were observed in leaf and clarified root extract inocula.

Last Modified: 3/30/2015
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