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United States Department of Agriculture

Agricultural Research Service

Title: Regulation of Metabolite Production by Biocontrol Agents in Liquid Culture: Potential Impact on the Bioefficacy and Phytotoxicity of Cell Harvests Formulated for Crop Protection

item Slininger, Patricia
item Van Cauwenberge, James
item Shea Wilbur, Maureen
item Bothast, Rodney

Submitted to: International Conference on Plant Growth Substances Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 18, 1995
Publication Date: N/A

Technical Abstract: Liquid culture technologies are being developed to mass produce fluorescent pseudomonads that can be applied as seed inoculants to biocontrol take-all, an important root disease of wheat. Nutritional and physical conditions of the culture environment were controlled in fermentors to regulate antibiotic and phytotoxin accumulations during production of Pseudomonas fluorescens 2-79. Culture harvests formulated in 0.5% methylcellulose wer applied as seed coatings, which provide an economical method of targeting the inoculation of emerging roots. Inoculum survival, as well as seed germination, take-all suppression, and seedling development observed in growth chamber bioassays were influenced by the liquid culture production conditions of the biocontrol agents.

Last Modified: 3/5/2015
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