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United States Department of Agriculture

Agricultural Research Service

Title: Regulation of Metabolite Production by Biocontrol Agents in Liquid Culture: Potential Impact on the Bioefficacy and Phytotoxicity of Cell Harvests Formulated for Crop Protection

Authors
item Slininger, Patricia
item Van Cauwenberge, James
item Shea Wilbur, Maureen
item Bothast, Rodney

Submitted to: International Conference on Plant Growth Substances Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 18, 1995
Publication Date: N/A

Technical Abstract: Liquid culture technologies are being developed to mass produce fluorescent pseudomonads that can be applied as seed inoculants to biocontrol take-all, an important root disease of wheat. Nutritional and physical conditions of the culture environment were controlled in fermentors to regulate antibiotic and phytotoxin accumulations during production of Pseudomonas fluorescens 2-79. Culture harvests formulated in 0.5% methylcellulose wer applied as seed coatings, which provide an economical method of targeting the inoculation of emerging roots. Inoculum survival, as well as seed germination, take-all suppression, and seedling development observed in growth chamber bioassays were influenced by the liquid culture production conditions of the biocontrol agents.

Last Modified: 4/21/2014
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