|Schoenbeck, Mark - UNIVERSITY OF MINNESOTA|
|Gregerson, Robert - UNIVERSITY OF MINNESOTA|
|Gantt, Stephen - UNIVERSITY OF MINNESOTA|
Submitted to: Plant Physiology
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1995
Publication Date: N/A
Technical Abstract: Mitogen-activated protein kinase (MAP kinase) constitutes a step in the phosphorylation cascade that leads to cell division in eukaryotes. Multiple forms of MAP kinase have been found in yeast, presumably mediating events of different signal pathways. We have cloned from an alfalfa (Medicago sativa) root nodule cDNA library a 2.4 kb cDNA (NMK8) which encodes a 420 amino acid polypeptide demonstrating a high degree of homology to other known MAP kinases. Regions of homology include a TDY variation of the TxY tripeptide target for stimulatory phosphorylation. Early results from poly(A)+ RNA blots suggest that though NMK8 transcripts are present in root, nodule, cotyledon, leaf, and stem, expression seems to be enhanced in the nodule. The predicted amino acid sequence of NMK8 shares 47 percent identity and 69 percent similarity to other reported alfalfa MAP kinase clones as determined by the BESTFIT program of the Genetics Computer Group, ,and has a molecular weight of approximately 46 kD.