|Imakawa, Kazuhiko - WRI, UNIV KS SCH MED|
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: May 25, 1995
Publication Date: N/A
Technical Abstract: Increased secretion of several uterine proteins, one of which has been identified as RBP, is temporally associated with glandular development in the neonatal porcine uterus. This study was designed to measure RBP mRNA in WC and M gilts during the ovary-independent (uterine gland formation) and ovary-dependent phase of development. In Exp. 1, 15 WC (3/d) and 20 M (4/d) )gilts were killed at d 1, 7, 14, 21, or 28. In Exp. 2, gilts were killed a d 42 (4 WC and 4 M), 70 (3 WC), or 85 (3 WC) to assess RBP mRNA levels following ovarian follicular development. Uteri were collected at necropsy; a 1-cm cross-section was fixed for in situ hybridization, and total RNA was extracted from the remainder. In Exp. 1, RBP mRNA was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR). In Exp. 2, RBP mRNA was detected by northern blot analysis. In WC gilts, RBP mRNA decreased (P < .05) from d 1 to 14 and 21 (107.6+/-13.7 vs 54.4+/-13.7 7and 34.7+/-13.7 pg/ug RNA, respectively). By d 28 RBP mRNA increased to values similar to d 1. In M gilts, RBP mRNA decreased (P < .05) from d 1 to 21 (118.8+/-16.8 vs 62.3+/-11.9 pg/ug RNA, respectively) and remained low to d 28. In Exp. 2, RBP mRNA was detectable by northern blot analysis by d 42 in M and by d 70 in WC gilts. In situ hybridization suggests that from d 1 to 28, RBP gene expression is occurring at low levels, and labeling was associated with the myometrium and stroma underlying the luminal epithelium. With the occurrence of follicles on ovaries of M (d 42) and WC gilts (d 70 and 85), labeling was detected in the luminal and glandular epithelium. These data indicate uterine RBP mRNA decreases initially in both WC and M gilts, and increases and localizes to the luminal and glandular epithelium following the onset of follicular activity.