ARS | Publication request: GUT AND LIVER GLUCOSE, LACTATE, AND INSULIN FLUX IN MATURE EWES DURING MESENTERIC OR ABDOMINAL VENA CAVA GLUCOSE INFUSION

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: February 21, 1995
Publication Date: N/A

Technical Abstract: Multicatheterized ewes were used to determine the effect of exogenous glucose infusion on net glucose, insulin, lactate, and oxygen flux by the portal-drained viscera (PDV) and liver of conscious sheep. Glucose was infused into either the mesenteric vein or abdominal vena cava at the same rate as the net hepatic release of glucose for 240 min, and net fluxes were determined every 30 min. Site of glucose infusion did not affect net PDV insulin or oxygen flux or the net liver glucose, insulin or oxygen flux. Net PDV glucose release was lower in the mesenteric (-3.6 +\- 1.4 mmol/h) than abdominal vena cava infusion (3.6 +\- 1.3 mmol/h). Net PDV insulin release differed over the infusion period. Net PDV lactate release and oxygen consumption did not differ over the infusion period. Net hepatic glucose release and oxygen consumption decreased during the infusion period. Net hepatic lactate and insulin release did not differ over the infusion period. Exogenous glucose infusion decreases hepatic aerobic metabolism, and the extent that net glucose and insulin fluxes are affected is dependent on length of infusion. Arterial insulin concentration (uU/mL) was linearly related to arterial glucose concentration (mM) (intercept = -2.7 +\- 2.5; slope = 7.3 +\- .7; R**2 = .45). Hepatic glucose release (mmol/h) was poorly related to arterial insulin concentration (intercept = 11.6 +\- 4.5; slope = .11 +\- .19; R**2 = .002) and to arterial glucose concentration (intercept = 1.4 +\- 7.2; slope = 3.6 +\- 2.0; R**2 = .02).