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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #326182
Research Project: Genetics, Breeding and Reproductive Physiology to Enhance Production of Catfish

Location: Warmwater Aquaculture Research Unit

Title: Draft genome sequence of Aeromonas hydrophila TN97-08

Author
item TEKEDAR, HASAN - Mississippi State University
item KUMRU, SALIH - Mississippi State University
item KARSI, ATTILA - Mississippi State University
item Waldbieser, Geoffrey - Geoff
item SONSTEGARD, TAD - Recombinetics, Inc
item Schroeder, Steven - Steve
item LILES, MARK - Auburn University
item GRIFFIN, MATT - Mississippi State University
item LAWRENCE, MARK - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/12/2016
Publication Date: 5/26/2016
Citation: Tekedar, H., Kumru, S., Karsi, A., Waldbieser, G.C., Sonstegard, T., Schroeder, S.G., Liles, M., Griffin, M., Lawrence, M. 2016. Draft genome sequence of Aeromonas hydrophila TN97-08. Genome Announcements. 4:e00436-16.

Interpretive Summary: Virulent strains of the bacteria Aeromonas hydrophila have been implicated in severe disease outbreaks in commercial catfish culture, leading to rapid and substantial losses of large catfish in affected ponds. A team of scientists from Mississippi State University, Auburn University, and USDA-ARS-Warmwater Aquaculture Research Unit used next-generation DNA sequencing technologies to produce a reference genome sequence assembly of the TN97-08 strain of A. hydrophila, which was isolated from diseased bluegill. Genomic comparison with other A. hydrophila strains revealed strain TN97-08 contained unique elements including a secretion system which is considered a virulence factor. Our ultimate aim is understand virulence and environmental adaptations of this species in order to develop diagnostics and therapeutants to reduce catfish production losses due to A. hydrophila infection.

Technical Abstract: Aeromonas hydrophila is an opportunistic Gram-negative species causing disease in fish and mammals. The genus Aeromonas affects a variety of aquatic organisms and lives in diverse aquatic ecosystems (1). There are 39 A. hydrophila genomes currently available in GenBank. In the current study, we report the draft genome sequence of strain TN97-08, which was isolated in 1997 from diseased bluegill (Lepomis macrochirus). The draft genome of A. hydrophila TN97-08 is comprised of 16 contigs and 5,087,310 bp. It contains 4,602 predicted genes, of which 4,445 are protein-coding. The annotated A. hydrophila TN97-08 genome was compared against the genomes of A. hydrophila ML09-119 and AL06-06. Results indicated A. hydrophila TN97-08 has 100 and 74 unique elements compared to strains ML09-119 and AL06-06, respectively. One of the unique features identified is a type VI secretion system, which is considered a virulence factor involved in the translocation of potential effector proteins into the host. Other unique elements include multidrug resistance efflux pumps, cobalt zinc cadmium resistance, stress response proteins, phage and prophage elements, and toxin-antitoxin replicon stabilization system elements. In contrast to the AL06-06 genome, TN97-08 does not carry any plasmids.