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Title: A Phocus on Phenotyping: opportunities and challenges in local and centralized trait evaluation from the VitisGen experience

Author
item Cadle-Davidson, Lance
item SACKS, GAVIN - Cornell University
item FENNELL, ANNE - South Dakota State University
item GADOURY, DAVID - Cornell University
item SUN, QI - Cornell University
item SCHWEITZER, PETER - Cornell University
item Londo, Jason
item Ledbetter, Craig
item CLARK, MATT - University Of Minnesota
item LUBY, JIM - University Of Minnesota
item HEMSTAD, PETER - University Of Minnesota
item HEGEMAN, ADRIAN - University Of Minnesota
item TEH, SOON LI - University Of Minnesota
item MANNS, DAVID - Cornell University
item BARBA, PAOLA - Cornell University
item HWANG, CHIN-FENG - Missouri State University
item SAPKOTA, SURYA - Missouri State University
item CHEN, LI-LING - Missouri State University
item FRESNEDO, JONATHAN - Cornell University
item YANG, SHANSHAN - Cornell University
item TAKACS, ELIZABETH - Cornell University
item REISCH, BRUCE - Cornell University

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/2015
Publication Date: 1/1/2016
Citation: Cadle Davidson, L.E., Sacks, G., Fennell, A., Gadoury, D., Sun, Q., Schweitzer, P., Londo, J.P., Ledbetter, C.A., Clark, M., Luby, J., Hemstad, P., Hegeman, A., Teh, S., Manns, D., Barba, P., Hwang, C., Sapkota, S., Chen, L., Fresnedo, J., Yang, S., Takacs, E., Reisch, B. 2016. A Phocus on Phenotyping: opportunities and challenges in local and centralized trait evaluation from the VitisGen experience. Plant and Animal Genome Conference. https://pag.confex.com/pag/xxiv/meetingapp.cgi/Paper/18722.

Interpretive Summary:

Technical Abstract: The integration of relevant genetic resources, robust phenotypes, and cutting-edge genotypic data is a challenge that individual scientists rarely overcome successfully. In the USDA-NIFA VitisGen project ( www.vitisgen.org ) for grapevine cultivar improvement, our research team has pursued a shared strategy 1) to maintain breeding germplasm locally, 2) to collect and analyze high-resolution genotypic data centrally, and 3) to collect and analyze phenotypic data both locally and in phenotyping centers specializing in fruit chemistry, low temperature response, and powdery mildew resistance. In the process of genotyping 28,000 individuals and collecting millions of phenotypic data points, challenges and unexpected opportunities arose from our approach. Here, we will ‘Phocus’ on Phenotyping, sharing our experiences and highlighting what seems to be working and not working in both local and central trait evaluation, based primarily on genetic analysis. These stories and results may facilitate both cultivar improvement and strategies for genetic analysis of traits.