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United States Department of Agriculture

Agricultural Research Service

Title: Differentiation of Sarcocystis Neurona from Eight Related Coccidia by Random Amplified Polymorphic DNA Assay

item Granstrom David, - GLUCK EQUINE RES CTR, KY
item Macpherson J M, - HLTH ANML LAB, CANADA
item Gajadhar Alvin A, - HLTH ANML LAB, CANADA
item Dubey, Jitender
item Tramontin Robert, - GLUCK EQUINE RES CTR, KY
item Stamper Shelby, - GLUCK EQUINE RES CTR, KY

Submitted to: American Association of Veterinary Parasitologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: July 11, 1994
Publication Date: N/A

Technical Abstract: The random amplified polymorphic DNA assay was used to compare four isolates of Sarcocystis neurona and eight species of coccidia from the genera Sarcocystis, Toxoplasma, or Eimeria. A single, common 550 bp DNA fragment was amplified from the DNA of each S. neurona isolate using a 16-nucleotide primer. Cross hybridization analyses among S. neurona isolates showed that DNA fragments had at least partial sequence homology. The primer generated several DNA fragments, including a 550 bp DNA fragment, from the related coccidia. DNA hybridization analyses indicated no sequence homology between these fragments and the 550 bp DNA fragment generated from S. neurona. The S. neurona 550 bp DNA fragment also did not hybridize with genomic blots of various related coccidia. These results suggest that the S. neurona DNA fragment may be useful as a species-specific probe.

Last Modified: 5/5/2015
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