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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #325971
Title: Preparation and application of new fluorescein-labeled fumonisins B1 in fluorescence polarization analysis technique

Author
item LIU, JI-HONG - Henan Agricultural University
item Maragos, Chris
item WANG, MIN - Henan Agricultural University
item YIN, HAI-YAN - Henan Agricultural University
item ZHANG, LING - Henan Agricultural University
item ZHANG, JUN-FENG - Henan Agricultural University
item WANG, HONG-QI - Henan Agricultural University

Submitted to: Trade Journal Publication
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/12/2015
Publication Date: 12/29/2015
Publication URL: http://handle.nal.usda.gov/10113/62421
Citation: Liu, J-H., Maragos, C.M., Wang, M., Yin, H-Y., Zhang, L., Zhang, J-F., Wang, H-Q. 2015. Preparation and application of new fluorescein-labeled fumonisins B1 in fluorescence polarization analysis technique. Journal of Food Safety & Quality. 6(12):1-7.

Interpretive Summary: Fumonisins are a group of compounds produced by certain fungi that commonly infest cereal grains. To keep these natural toxins out of the food and feed supplies, commodities are routinely screened for their presence. This research, conducted by scientists at the Henan Academy of Agricultural Sciences (Henan, China) in cooperation with the Mycotoxin Prevention and Applied Microbiology Research Unit (Peoria, IL), was conducted in order to improve a reagent used in a type of toxin screening assay: fluorescence polarization immunoassay. A less expensive alternative was developed to the existing reagent, reducing the costs for the detection kits and potentially reducing the costs for monitoring for this group of toxins.

Technical Abstract: Objective: To prepare a new fluorescent tracer against common mycotoxins such as fumonisin B1 in order to replace 6-(4,6-Dichlorotriazinyl) aminofluorescein (6-DTAF), an expensive marker, and to develop a technique for quick detection of fumonisin B1 based on the principle of fluorescence polarization. Methods: A one-pot method was used to synthesize a 5-(4,6-Dichlorotriazinyl) aminofluorescein (5-DTAF) fluorescent marker. To detect fumonisins, a fluorescence polarization technique based on the 5-DTAF derivative was established. Results: The fluorescence polarization assays based on the 5-DTAF and 6-DTAF derivatives of fumonisins B1 had almost the same analytical capability and the value of IC50. Conclusion: A new fluorescent marker of fumonisins B1 was successfully synthesized by using 5-DTAF, and a rapid assay for fumonisin B1 using fluorescence polarization technology was developed. Moreover, the fluorescence polarization assay based on the 5-DTAF marker had almost the same analysis capability compared to that of the 6-DTAF fluorescent marker, but cut the analysis cost in half.