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United States Department of Agriculture

Agricultural Research Service

Title: Lymphocyte and Hormone Responses after Antigen Stimulation in B-G Mhc Congenic Lines Selected for Antibody Response to Srbc

Authors
item Siegel H S, - PENN STATE UNIVERSITY
item Mashaly M M, - PENN STATE UNIVERSITY
item Briles W E, - NORTHERN ILLINOIS UNIV
item Lillehoj, Hyun
item Siegel P B, - VIRGINIA POLYTECHNIC UNIV
item Dunnington E A, - VIRGINIA POLYTECHNIC UNIV

Submitted to: Congress International Society Develop Comparative Immunology
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 1994
Publication Date: N/A

Technical Abstract: In two experiments, congenic lines of chickens that were selected for high (H) and low (L) antibody response to SRBC and typed for B-G MHC genotypes (GTP) B13B13, B13B21, and B21B21, were immunized with SRBC or PBS. Blood and spleens were processed for % B+, CD4+ and CD8+ lymphocytes by flow cytometry at 0, 3, 9 and 24 hr post-antigen (pAg) injection. Plasma T3, T4 and ACTH were determined by RIA. There were significant time pAg changes in cell percentages and hormone levels. CD4+ cells in blood and spleen were significantly higher in the B21B21 than in the B13B13 GTP, with the B13B13 intermediate, suggesting that the MHC influence is through helper T-cell activity. Plasma ACTH levels were highest in the B21B21 GTP and in SRBC-treated birds, but only in the L lines, resulting in significant GTP and Ag x line interactions. These results indicate the MHC and Ag-related hormone responses are influenced by genetic background.

Last Modified: 11/27/2014
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