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ARS Home » Pacific West Area » Wapato, Washington » Temperate Tree Fruit and Vegetable Research » Research » Publications at this Location » Publication #319832
Title: Complete genomic characterization of potato mop-topvirus isolate from the United States

Author
item RAMESH, S - Washington State University
item RAIKHY, G - Washington State University
item Brown, Charles
item Whitworth, Jonathan
item PAPPU, HANU - Washington State University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/25/2014
Publication Date: 10/8/2014
Citation: Ramesh, S.V., Raikhy, G., Brown, C.R., Whitworth, J.L., Pappu, H. 2014. Complete genomic characterization of potato mop-topvirus isolate from the United States. Archives of Virology. 159:3427-3422.

Interpretive Summary: Among the pathogens of plants and animals viruses are among the simplest. They usually consist of strip of RNA about 10 thousand units long. The information is little mor complicated than fortune cookie. During the disease process they take over certain functions in the cell, sometimes move between cells or long distances in the entire plant. They can cause mild symptoms or devastate the plant. The potato mop top virus is called such because certain potatoes are foreshortened and look like brushes or mops on the tops. The virus is of such concern because it is spread around by a damaging fungus that we cannot control. The virus causes necrotic lesions which are unattractive and make the crop unsalable for fresh market and processing. Since there is so little remedy available knowing the code expressed in its simple strip ofInstruction is of potential use in the future strategies to control the virus. The PTMV of North America has a genetic code most closely resembling European codes. Potato Mop Top Virus originally came from South America and has diverged hardly at all since it migrated neither in Europe nor in North America.

Technical Abstract: Potato mop-top virus (PMTV; family Virgaviridae)was reported recently in the Pacific Northwestern USA. To better understand the genetic diversity of thisvirus, the complete genome of an isolate from WashingtonState (WA), USA, was characterized. Sequence comparisons of the WA isolate with other known sequences revealed that the RNA-Rep-encoded RdRp protein and the RNA-CP-encoded coat protein displayed [99 % amino acid sequence identity to those of two Nordic (RdRp) and several European and North American isolates (CP),respectively. The RNA-TGB-encoded TGB 1 and TGB 3 protein sequences had[99 % amino acid sequence identityto the corresponding proteins of Czech and Danish isolates, whereas the TGB 2 protein is identical to those of Colombian isolates. Phylogenetic analysis of the viral genes of the WA isolate reflected the close relationship between WA and European isolates. RFLP analysis ofcorresponding DNA of RNA TGB and RNA CP revealedthat the WA isolate has the RNA TGB-II and RNA CP-B types, which are prevalent in Europe and other parts of world. This is the first report of the complete genome characterization of PMTV from the Americas