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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food Processing and Sensory Quality Research » Research » Publications at this Location » Publication #317551
Title: Soy Glyceollins Regulate Transcript Abundance in the Female Mouse Brain

Author
item BAMJI, SANAYA - University Of Louisville
item PATEL, DHARTI - University Of Louisville
item PAGE, ROBERT - College Of St Benedict & St John'S University
item SANDERS, ALEXIA - University Of Louisville
item GAMBRELL, CAITLIN - University Of Louisville
item ALVAREZ, ALEJANDRO - University Of Louisville
item NAIK, KUNTESH - University Of Louisville
item RAGHAVAN, ASHWIN - University Of Louisville
item BUROW, MATTHEW - Tulane University
item Boue, Stephen
item KLINGE, CAROLYN - University Of Louisville
item IVANOVA, MARGARITA - University Of Louisville
item CORBITT, CYNTHIA - University Of Louisville

Submitted to: Functional and Integrative Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/7/2015
Publication Date: 5/8/2015
Citation: Bamji, S.F., Patel, D., Page, R.B., Sanders, A., Gambrell, C., Alvarez, A.R., Naik, K., Raghavan, A.M., Burow, M., Boue, S.M., Klinge, C.M., Ivanova, M., Corbitt, C. 2015. Soy Glyceollins Regulate Transcript Abundance in the Female Mouse Brain. Functional and Integrative Genomics. 15:549-561.

Interpretive Summary: Glyceollins, produced by soy plants in response to stress, have anti-estrogenic activity in breast and ovarian cancer cell lines in vitro and in vivo. In addition to known anti-estrogenic effects, the glyceollins exhibit mechanisms of action not involving estrogen receptor signaling. To date, the effects of glyceollin on gene expression in the brain are unknown. For this study, we implanted estradiol or placebo slow-release pellets into ovariectomized mice followed by 11 days of exposure to glyceollin or vehicle using injections. We then performed a microarray on total RNA extracted from whole brain hemispheres and identified differentially expressed genes. In total, we identified 33 differentially expressed genes with a significant estradiol main effect, 5 differentially expressed genes with a significant glyceollin main effect, 74 differentially expressed genes with significant glyceollin and estradiol main effects (but no significant interaction term), and 167 differentially expressed genes with significant interaction terms. Collectively, our results suggest that whether glyceollin acts in the brain through estrogen receptor-dependent or estrogen receptor-independent mechanisms depends on the target gene.

Technical Abstract: Glyceollins (Gly), produced by soy plants in response to stress, have anti-estrogenic activity in breast and ovarian cancer cell lines in vitro and in vivo. In addition to known anti-estrogenic effects, Gly exhibits mechanisms of action not involving estrogen receptor (ER) signaling. To date, effects of Gly on gene expression in the brain are unknown. For this study, we implanted 17-ß estradiol (E2) or placebo slow-release pellets into ovariectomized CFW mice followed by 11 days of exposure to Gly or vehicle i.p. injections. We then performed a microarray on total RNA extracted from whole brain hemispheres and identified differentially expressed genes (DEGs) by a 2 x 2 factorial ANOVA with an FDR = 0.20. In total, we identified 33 DEGs with a significant E2 main effect, 5 DEGs with a significant Gly main effect, 74 DEGs with significant Gly and E2 main effects (but no significant interaction term), and 167 DEGs with significant interaction terms. Clustering across all DEGs revealed that transcript abundances were similar between the E2+Gly and E2-only treatments. However, gene expression after Gly-only treatment was distinct from both of these treatments and was generally characterized by higher transcript abundance. Collectively, our results suggest that whether Gly acts in the brain through ER-dependent or ER-independent mechanisms depends on the target gene.