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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #315024
Title: Generation of Newcastle diease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoprotein gB or gD as dual vaccines

Author
item ZHAO, WEI - Beijing Center For Diseases Prevention And Control
item Spatz, Stephen
item Zsak, Laszlo
item Yu, Qingzhong

Submitted to: Vaccine Design: Methods and Protocols
Publication Type: Book / Chapter
Publication Acceptance Date: 8/28/2015
Publication Date: 5/1/2016
Publication URL: http://handle.nal.usda.gov/10113/62588
Citation: Zhao, W., Spatz, S.J., Zsak, L., Yu, Q. 2016. Generation of Newcastle diease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoprotein gB or gD as dual vaccines. In: Thomas, S.,editor. Vaccine Design: Methods and Protocols. volume 2. New York, NY: Springer Science+Business Media. p.89-101.

Interpretive Summary: Infectious laryngotracheitis (ILT) and Newcastle disease (ND) are two of the most economically important infectious diseases of poultry. Vaccination combined with strict biosecurity practices has been the recommended strategy for controlling ILT and ND outbreaks. However, the current commercial ILT vaccines are either not safe or less effective. Therefore, there is a need to develop safer and more efficacious ILTV vaccines. In this book chapter, we describe the generation of the Newcastle disease virus (NDV) LaSota vaccine strain-based recombinant viruses expressing an immunogenic protein of infectious laryngotracheitis virus (ILTV) as dual vaccines against ND and ILT using cutting edge technology.

Technical Abstract: Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens caused by infection of infectious laryngotracheitis virus (ILTV). The current commercial ILT vaccines are either not safe or less effective. Therefore, there is a pressing need to develop safer and more efficacious ILTV vaccines. Newcastle disease (ND), caused by infection with virulent Newcastle disease virus (NDV), is one of the most serious infectious diseases of poultry. The NDV LaSota strain, a naturally occurring low-virulence NDV strain, has been routinely used as a live vaccine throughout the world. This chapter describes the generation of the Newcastle disease virus (NDV) LaSota vaccine strain-based recombinant viruses expressing the glycoprotein B (gB) or D (gD) of ILTV as dual vaccines against ND and ILT using reverse genetics technology.