Author
SHEN, X.K. - Sichuan University | |
MA, L.X. - Sichuan University | |
ZHONG, S.F. - Sichuan University | |
LIU, NA - Sichuan University | |
ZHANG, M. - Sichuan University | |
CHEN, W.Q. - Sichuan University | |
ZHOU, Y.L. - Sichuan University | |
LI, H.J. - Beijing Academy Of Agricultural Sciences | |
ZHANG, Z.J. - Shanxi University | |
LI, X - Sichuan University | |
Bai, Guihua | |
ZHANG, H.Y. - Sichuan University | |
TAN, F.Q. - Sichuan University | |
REN, Z.L. - Sichuan University | |
LUO, P.G. - Sichuan University |
Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 12/17/2014 Publication Date: 1/4/2015 Citation: Shen, X., Ma, L., Zhong, S., Liu, N., Zhang, M., Chen, W., Zhou, Y., Li, H., Zhang, Z., Li, X., Bai, G., Zhang, H., Tan, F., Ren, Z., Luo, P. 2015. Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. Theoretical and Applied Genetics. doi: 10.1007/s00122-014-2449-x. Interpretive Summary: Powdery mildew (PM) is a destructive foliar disease in wheat. To improve wheat resistance to PM, a chromosome fragment from a wheat wild relative, Thinopyrum intermedium, was transferred to a Chinese common wheat line, L962. The alien fragment confers resistance to multiple Chinese PM isolates. Genetic analysis using the F1, F2, and F2:3 populations derived from the cross of L983/L962 indicated that resistance was controlled by a single dominant gene. This gene was mapped to the short arm of chromosome 2B and is a novel PM resistance gene designated as PmL962. Two flanking markers to the gene, Xwmc314 and BE443737 at 5.83 cM apart, were identified. The flanking markers have potential applications in marker-assisted breeding. Technical Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F1, F2, and F2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737 at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding. |