Author
KHERA, PAWAN - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
PANDEY, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
MALLIKARJUNA, NALINI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
SRISWATHI, MANDA - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
ROORKIWAL, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
JANILA, PASUPULETI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
SHILPA, KRISHNA - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
SUDINI, HARIKISHAN - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India | |
Guo, Baozhu | |
VARSHNEY, RAJEEV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 11/8/2014 Publication Date: 11/10/2014 Citation: Khera, P., Pandey, M.K., Mallikarjuna, N., Sriswathi, M., Roorkiwal, M., Janila, P., Shilpa, K., Sudini, H., Guo, B., Varshney, R.K. 2014. Development of introgression lines and advanced backcross QTL analysis for disease resistance, oil quality and yield component traits in peanut. Meeting Abstract. Advanced in Arachis through Genomics and Biotechnology (AAGB) meeting, November 10-14, 2014, Savannah, Georgia. Interpretive Summary: Technical Abstract: Ploidy difference between wild Arachis species and cultivated genotypes hinder transfer of useful alleles for agronomically important traits. To overcome this genetic barrier, several synthetics have been developed at ICRISAT. Furthermore, two synthetic amphidiploids viz., ISATGR 1212 (A. duranensis ICG 8123 × A. ipaensis ICG 8206) and ISATGR 265-5A (A. kempff-mercadoi ICG 8164 × A. hoehnei ICG 8190) have been used to generate two advanced backcross (AB) populations. The AB populations namely AB1 (ICGV 91114 × ISATGR 1212) and AB2 (ICGV 87846 × ISATGR 265-5A) have been genotyped with DArT and SSR markers. Based on these marker genotyping data, 258 (253 DArT and 5 SSR) and 1043 (1034 DArT and 9 SSR) loci have been mapped on 20 and 19 linkage groups covering a total map length of 1415.7 cM and 1500.8 cM with map density of 5.49 and 1.44 cM for AB1 and AB2 populations, respectively. These populations have also been phenotyped for disease resistance (late leaf spot, rust and peanut bud necrosis), oil quality (oleic and linoleic acid ratio, saturated and unsaturated fatty acids), and yield components (pod yield, 100 seed weight, sound mature kernels and shelling percentage). Quantitative trait locus (QTL) analysis using above mentioned genotyping and phenotyping identified 15 QTLs (PV 6.68-14.84%) in AB1 population while 34 QTLs (PV 6.9-67.8%) in AB2 population for disease resistance, oil quality and yield component traits. The AB-QTL approach facilitated simultaneous identification of QTLs and introgression of wild genomic regions associated with traits of interest into the cultivated gene pool of peanut. |