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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC CONTROL OF FUSARIUM MYCOTOXINS TO ENHANCE FOOD SAFETY

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: Using barley genomics to develop Fusarium head blight resistant wheat and barley

Authors
item Muehlbauer, Gary -
item Li, Xin -
item Shin, Sanghyun -
item Dill-Macky, Ruth -
item Berthiller, Franz -
item Clemente, Thomas -
item McCormick, Susan
item Kumlehn, Jochen -
item Adam, Gerhard -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 2, 2014
Publication Date: July 2, 2014
Citation: Muehlbauer, G., Li, X., Shin, S., Dill-Macky, R., Berthiller, F., Clemente, T., McCormick, S.P., Kumlehn, J., Adam, G. 2014. Using barley genomics to develop Fusarium head blight resistant wheat and barley [abstract]. American Barley Researchers Workshop.

Technical Abstract: Fusarium head blight, caused by Fusarium graminearum, is a major problem for wheat and barley growers. During infection, F. graminearum produces trichothecene mycotoxins (e.g., deoxynivalenol or DON) that increases fungal virulence and reduces grain quality and yield. Previous work in Arabidopsis showed that an UDP-glucosyltransferases (DOGT1) inactivates DON by conjugating it with a glucoside group to DON-3-O-glucoside (D3G). Using genomics approaches we identified a barley UDP-glucosyltransferase (HvUGT13248) that exhibited DON resistance in yeast and Arabidopsis via conjugation of DON to D3G. Transgenic wheat in the Bobwhite and CBO37 backgrounds overexpressing HvUGT13248 exhibited high levels of type II resistance in the greenhouse and Type I resistance in the field compared to the nontransgenic controls. In addition, field grown transgenic plants compared to the nontransgenic controls exhibited a lower DON concentration. The mechanism of resistance in these transgenic plants appears to be the rapid conversion of DON to D3G. Noteworthy, these plants also exhibited resistance to F. graminearum strains that produce the trichothecene nivalenol, indicating that HvUGT13248 has a broad spectrum of activity. Transgenic barley in the Golden Promise background overexpressing HvUGT13248 have been developed and we will present the screening results. To facilitate screening in the field, development of backcross lines in the Rasmusson background is in process.

Last Modified: 10/25/2014
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