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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Peanut Germplasm with Improved Yield, Oil Quality, and Tolerance to Biotic and Abiotic Stresses

Location: Crop Genetics and Breeding Research

Title: Development and phenotyping of recombinant inbred line (RIL) populations for peanut (Arachis hypogaea)

Authors
item Holbrook, C
item Isleib, T -
item Ozias-Akins, P -
item Chu, Y -
item Knapp, S -
item Tillman, B -
item Guo, Baozhu
item Gill, R -
item Burow, M -

Submitted to: Peanut Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 15, 2014
Publication Date: March 20, 2014
Citation: Holbrook Jr, C.C., Isleib, T.G., Ozias-Akins, P., Chu, Y., Knapp, S.J., Tillman, B.L., Guo, B., Gill, R., Burow, M.D. 2014. Development and phenotyping of recombinant inbred line (RIL) populations for peanut (Arachis hypogaea). Peanut Science. 40:89-94.

Interpretive Summary: Peanut has lagged behind other crop species in the development and use of genetic markers for marker-assisted selection (MAS). MAS can be used to greatly improve the effectiveness and efficiency of plant breeding for variety development. Development and phenotypic evaluation of recombinant inbred line (RIL) populations of peanut, along with molecular genotyping, will be essential for marker development. The primary objectives of this research were to develop 16 structured RIL populations that can be used by the peanut research community, and to begin high-resolution phenotyping of these populations. Parents were selected to attempt to maximize genetic diversity while meeting practical breeding objectives. First, two modern runner cultivars (Tifrunner and Florida-07) were selected as common parents because runner cultivars account for about 80% of the production in the US. Second, the eight unique parents were selected to supply diversity across market classes and botanical varieties and are donors of favorable alleles for enhancing drought tolerance and resistance to most important disease of peanut in the U.S. In-depth phenotyping and genotyping of these populations is likely to result in the development of markers that can be deployed by breeding programs for the development of improved varieties.

Technical Abstract: The identification of molecular markers for economically significant traits should greatly improve the speed and efficiency of all peanut(Arachis hypogaea L.) breeding programs. Development and phenotypic evaluation of recombinant inbred line (RIL) populations of peanut, along with molecular genotyping, will be essential for marker development. The primary objectives of this research were to develop 16 structured RIL populations that can be used by the peanut research community, and to begin high-resolution phenotyping of these populations. Crosses were made using a 2 x 8 (common x unique) factorial nested association mapping design. Parents were selected to attempt to maximize genetic diversity while meeting practical breeding objectives. First, two modern runner cultivars (Tifrunner and Florida-07) were selected as common parents because runner cultivars account for about 80% of the production in the U.S. Second, the eight unique parents were selected to supply diversity across market classes and botanical varieties and are donors of favorable alleles for enhancing drought tolerance and resistance to most important disease of peanut in the US. The eight unique parents are N08082olJCT (a Bailey derived high oleic breeding line), C76-16, NC 3033, SPT 06-06, SSD 6 (PI 576638), OLin, New Mexico Valencia A, and Florunner. The 16 populations were advanced using summer and winter nurseries. Input from multiple disciplines has resulted in a list of populations and traits that should be examined first, and seed increase has begun to provide the community with material for extensive phenotyping. In-depth phenotyping and genotyping of these populations is likely to result in the development of markers that can be deployed by breeding programs for the development of improved cultivars.

Last Modified: 11/25/2014
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