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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium sp. strain RPT-4213

Authors
item LIU, SIQING
item BISCHOFF, KENNETH
item LEATHERS, TIMOTHY
item QURESHI, NASIB
item RICH, JOSEPH
item HUGHES, STEPHEN

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 24, 2014
Publication Date: N/A

Technical Abstract: A novel Clostridium sp. strain RPT-4213 was found producing butyrate under strict anaerobic conditions. This strain produced 9.47 g L-1 butyric acid from MRS media (0.48 g/g glucose). RPT-4213 was also used to ferment dilute acid pretreated hydrolysates including wheat straw (WSH), corn fiber (CFH), corn stover (CSH), rice hull (RHH), and switchgrass (SGH). Results indicated that 50% WSH with a Clostridia medium (Ct) produced the most butyric acid (8.06 g L-1, 0.46 g/g glucose), followed by 50% SGH with Ct (6.01 g L-1, 0.44 g/g glucose), however, 50% CSH Ct showed growth inhibition. RPT-4213 was then used in pH-controlled bioreactor fermentations using 60% WSH and SGH, with a dilute (0.5 x) Ct medium, resulting 9.87 g L-1 butyric acid in WSH (yield 0.44 g/g) and 7.05 g L-1 butyric acid in SGH (yield 0.42 g/g). The titer and productivity could be improved through process engineering.

Last Modified: 9/29/2014
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