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United States Department of Agriculture

Agricultural Research Service

Research Project: MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES

Location: Tick and Biting Fly Research

Title: Rdl-containing Fragment of GABA(A) from the Horn Fly, Haematobia Irritans, Susceptible Genotype

Authors
item Domingues, L -
item Guerrero, Felix
item Foil, Lane -

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: December 17, 2012
Publication Date: June 17, 2013
Citation: Domingues, L.N., Guerrero, F., Foil, L.D. 2013. Rdl-containing Fragment of GABA(A) from the Horn Fly, Haematobia Irritans, Susceptible Genotype. National Center for Biotechnology Information (NCBI). http://www.ncbi.nlm.nih.gov. Accession Number KC346253.

Interpretive Summary: The horn fly, Haematobia irritans (L.), is a significant economic pest of cattle found throughout Europe, North Africa, Asia Minor, and the Americas. The major means of controlling the horn fly is through insecticides. A cyclodiene-containing ear tag product has been recently introduced and we are developing DNA-based methods to detect target site resistance to cyclodienes. Cyclodiene insecticides inhibit chloride ion transport by targeting a receptor known as the gamma-aminobutyric acid subtype A (GABAA) receptor. Target site resistance to cyclodienes (termed Rdl, resistance to dieldrin) in several species of insects is associated with a single amino acid substitution in a GABAA receptor locus. We have cloned and sequenced a 215 bp fragment from the horn fly GABAA receptor that encompasses the location of the Rdl gene mutation in cyclodiene resistant insects.

Technical Abstract: The horn fly, Haematobia irritans (L.), is a significant economic pest of cattle found throughout Europe, North Africa, Asia Minor, and the Americas. The major means of controlling the horn fly is through applications of chemicals with insecticidal activity. A cyclodiene-containing ear tag product has been recently introduced. Cyclodiene insecticides inhibit chloride ion transport regulated by the gamma-aminobutyric acid subtype A (GABAA) receptor and resistance to cyclodienes (termed Rdl) is associated with a single amino acid substitution in a GABAA receptor locus. We have cloned and sequenced a 215 bp fragment from the horn fly GABAA that encompasses the location of the Rdl gene mutation in cyclodiene resistant insects.

Last Modified: 11/23/2014
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