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United States Department of Agriculture

Agricultural Research Service

Research Project: Using Genetic Approaches to Reduce Crop Losses in Rice Due to Biotic and Abiotic Stress

Location: Dale Bumpers National Rice Research Center

Title: Using the rice diversity panel 1 to develop novel germplasm for breeding

Authors
item Eizenga, Georgia
item McClung, Anna
item McClung, Anna
item Wright, Mark -
item Greenberg, Anthony -
item Cobb, Joshua -
item Jung, Janelle -
item Clark, Randy -
item Singh, Namrata -
item Agosto-Perez, Fernando -
item Declerck, Genevieve -
item Shakiba, Ehsan -
item Anh, Sang-Nag -
item Kochian, Leon
item Mezey, Jason -
item Mccouch, Susan -

Submitted to: International Rice Genetics Symposium
Publication Type: Abstract Only
Publication Acceptance Date: July 31, 2013
Publication Date: November 1, 2013
Citation: Eizenga, G.C., Mcclung, A.M., Wright, M.H., Greenberg, A.J., Cobb, J.N., Jung, J.K., Clark, R.T., Singh, N., Agosto-Perez, F.J., Declerck, G.A., Shakiba, E., Anh, S., Kochian, L.V., Mezey, J.G., Mccouch, S.R. 2013. Using the rice diversity panel 1 to develop novel germplasm for breeding. International Rice Genetics Symposium. 7th International Rice Genetics Symposium Program and Abstract Book. pg 161-162.

Technical Abstract: The Rice Diversity Project aims to explore the relationship between genotypic and phenotypic variation in a panel of rice (Oryza sativa) landraces, elite cultivars, and wild ancestors to utilize this broad range of natural variation in rice improvement. The ‘Rice Diversity Panel 1’ (RDP1) is composed of 421 diverse accessions and represents the aus, indica, aromatic (Group V), tropical japonica and temperate japonica subpopulations. The RDP1 was phenotyped for plant, panicle, seed, grain quality and root traits, aluminum tolerance, seedling cold tolerance, and micronutrient (ion) concentrations. In parallel, 96 O. rufipogon accessions were characterized for many of the same phenotypic traits. Initially, accessions were genotyped with a fixed array of 36,901 SNPs and genome-wide association (GWA) mapping demonstrated the genetic architecture of complex traits differed within the subpopulations for most of the aforementioned phenotypes. Recently, the RDP1 was genotyped using a high density rice array (HDRA) that generated ~450,000 SNPs, and GWA analyses are underway. To validate the GWA results, tropical-temperate japonica RIL and MAGIC populations are being developed. To further explore the genetic potential of the wild ancestor, O. rufipogon, three diverse accessions were crossed with two elite cultivars, IR64 (indica) and Cybonnet (tropical japonica). From these crosses, six sets (libraries) of chromosome segment substitution lines (CSSLs) are being developed via marker assisted backcrossing. The CSSLs will be evaluated for a wide array of traits, including yield and yield response to stress, to better understand the genetic potential of O. rufipogon as a source of variation for improving the performance of elite rice cultivars.

Last Modified: 12/25/2014
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