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United States Department of Agriculture

Agricultural Research Service

Research Project: Biorefining Processes

Location: Bioproducts Research

Title: Engineering yeast for the expression and secretion of cellulase cocktails

Authors
item Batt-Throne, Sarah
item Wan, David
item Doi, Roy -
item Wong, Dominic

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 30, 2013
Publication Date: N/A

Technical Abstract: Enzyme systems that digest the cellulose in plant cell walls have potential value in the biorefining of renewable feedstocks such as crop residues, straws, and grasses to biofuels and other bioproducts. The bacterium Clostridium cellulovorans is a useful source of biomass-degrading enzymes because it produces cellulase systems that degrade crystalline cellulose. The genes of three C. cellulovorans cellulases were individually cloned into the model eukaryotic organism Saccharomyces cerevisiae. Active endoglucanase, exoglucanase, and '-glucosidase enzymes were constitutively expressed and secreted. The three cellulases were also co-cloned into S. cerevisiae, and PCR was used to confirm the presence of all three genes in engineered yeast clones. Enzyme activity was detected by analyzing the degradation of CMC in yeast cultures. This cellulase cocktail is being studied as a prospective component of enzyme systems for the conversion of cellulosic biomass to fermentable sugars.

Last Modified: 10/23/2014
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