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United States Department of Agriculture

Agricultural Research Service

Research Project: Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry

Location: Endemic Poultry Viral Diseases Research Unit

Title: Molecular characterization of turkey enteric coronaviruses circulating in the United States in 2012

Authors
item Day, James
item Gonder, Eric -
item Jennings, Shannon -
item Rives, David -
item Tilley, Becky -
item Wooming, Brian -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 21, 2012
Publication Date: N/A

Technical Abstract: The use of molecular diagnostic assays has allowed ongoing periodic monitoring of United States turkey flocks for suspected viral enteric pathogens such as reovirus, rotavirus, parvovirus, and astrovirus. Beginning in early 2012, monitoring of commercial turkey flocks in the Southeastern United States via a real-time reverse-transcriptase polymerase chain reaction assay (RT-PCR) revealed a sharp increase in the number of turkey enteric coronavirus (TCoV) positive flocks. Over the course of 2012, numerous observations of suspected turkey coronavirus enteritis were reported from multiple integrators; many of these flocks experiencing enteric disease were positive for TCoV via the RT-PCR assay. This report will focus on the subsequent sequence analysis of the TCoV spike glycoprotein gene and the direct phylogenetic comparisons of TCoV isolates from North Carolina and Arkansas, and will correlate the molecular data with observations from the field and serological data.

Last Modified: 12/21/2014
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