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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #295761
Title: Potato transformation and potato cyst nematode infection on potato plantlets in tissue culture

Author
item Chronis, Demosthenis
item CHEN, SHIYAN - Cornell University
item LANG, PING - Cornell University
item TRAN, TIEN - Cornell University
item Wang, Xiaohong

Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/28/2013
Publication Date: 1/5/2014
Citation: Chronis, D.N., Chen, S., Lang, P., Tran, T., Wang, X. 2014. Potato transformation and potato cyst nematode infection on potato plantlets in tissue culture. Bio-protocol. Available: www.biio-protocol.org/wenzhang.aspx?id=1017.

Interpretive Summary: We have described two protocols for generating transgenic potato plants and for evaluating tissue-cultured potato plants for infection by potato cyst nematodes. These protocols are useful for the study of the interactions between potato cyst nematodes and potato.

Technical Abstract: These two protocols describe the methods for generating transgenic potato plants and for evaluating potato cyst nematode (Globodera rostochiensis and G. pallida) infection on potato plantlets in tissue culture. These methods are useful tools that can be used in the study of the interactions between potato cyst nematodes and their host plant of potato.