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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate

Authors
item Hughes, Stephen
item Cox, Elby
item Kurtzman, Cletus
item Bischoff, Kenneth
item Liu, Siqing
item Cote, Gregory
item Rich, Joseph
item Bang, Sookie -
item Pinkleman, Rebecca -
item Riano-Herrera, Nester -
item Rodriguez-Valencia, Nelson -
item Lopez-Nunez, Juan -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 7, 2013
Publication Date: May 7, 2013
Citation: Hughes, S.R., Cox, E.J., Kurtzman, C.P., Bischoff, K.M., Liu, S., Cote, G.L., Rich, J.O., Bang, S.S., Pinkleman, R., Riano-Herrera, N.M., Rodriguez-Valencia, N., Lopez-Nunez, J.C. 2013. Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate [abstract]. Center for Process Analysis and Control.

Technical Abstract: The yeast K. marxianus has advantages over the most commonly used industrial ethanologen, Saccharomyces cerevisiae, such as the ability to grow at 47°C, to produce ethanol at temperatures above 40°C, and to grow on a wide variety of substrates, including starch, sucrose, pectins, and cellulosic biomass, that make it a promising candidate for development as a versatile, thermotolerant, industrial ethanologen. To improve its growth and ethanol yield at elevated temperature under microaerophilic conditions, wild-type K. marxianus NRRL Y 1109 was irradiated with UV C and the irradiated cultures were selected for growth at elevated temperature using automated protocols on a robotic platform for picking and spreading the cultures and for processing the resulting plates. Two mutant strains were isolated that grew aerobically on glucose at 47°C and anaerobically at 46°C on a wide variety of substrates that are constituents of biomass materials commonly considered feedstocks for renewable fuels. These strains gave higher ethanol yields than wild-type using either glucose (0.51 and 0.43 g ethanol/ g glucose, respectively; P=0.014) or galacturonic acid (0.48 and 0.34 g ethanol/ g galacturonic acid, respectively; P<0.00001) as substrate at 46°C.

Last Modified: 10/1/2014
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