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United States Department of Agriculture

Agricultural Research Service

Research Project: Biology and Control of Human Pathogens on Fresh Produce

Location: Produce Safety and Microbiology Research

Title: Serotype assignment by sero-agglutination, ELISA, and PCR

Author
item Gorski, Lisa

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: October 23, 2013
Publication Date: May 20, 2014
Citation: Gorski, L.A. 2014. Serotype assignment by sero-agglutination, ELISA, and PCR.In: Jordan, K., Fox, E.M., Wagner, M., editors. Methods in Molecular Biology. New York, NY: Humana Press. p. 41-61.

Technical Abstract: For assessing isolates of Listeria monocytogenes serotype designation is the foremost subtyping method used. Traditionally serotyping has been done with agglutination reactions. In the last decade alternative serotyping methods were described using Enzyme Linked Immunosorbent Assay(ELISA)and Polymerase Chain Reaction(PCR). Herein are described the three methods, and the advantages and disadvantages of the three.

Last Modified: 10/23/2014
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