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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT AND GENETIC CHARACTERIZATION OF AGRICULTURAL AND BIOTECHNOLOGICAL MICROBIAL RESOURCES

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: Taxonomic evaluation of Streptomyces albus and related species using multilocus sequence analysis

Authors
item LABEDA, DAVID
item PRICE, NEIL
item Doroghaz, James -
item Ju, Ku-San -
item Metcalf, William -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 25, 2013
Publication Date: July 25, 2013
Citation: Labeda, D.P., Price, N.P., Doroghaz, J.R., Ju, K., Metcalf, W.W. 2013. Taxonomic evaluation of Streptomyces albus and related species using multilocus sequence analysis [abstract]. Federation of European Microbiological Societies.

Technical Abstract: In phylogenetic analyses of the genus Streptomyces using 16S rRNA gene sequences, Streptomyces albus subsp. albus NRRL B-1811T formed a cluster with 5 other species having identical or nearly identical 16S rRNA gene sequences. Moreover, the morphological and physiological characteristics of these other species, including S. almquistii NRRL B-1685T, S. flocculus NRRL B-2465T, S. gibsonii NRRL B-1335T and S. rangoonensis NRRL B-12378T are quite similar. This cluster is of particular taxonomic interest because Streptomyces albus is the type species of the genus Streptomyces. The related strains were subjected to multilocus sequence analysis (MLSA) utilizing partial sequences of the house-keeping genes atpD, gyrB, recA, rpoB, and trpB, as well as confirmation of previously reported phenotypic characteristics. The 6 strains formed a coherent cluster supported by a 100% bootstrap value in phylogenetic trees generated from sequence alignments prepared by concatenating the sequences of the house-keeping genes and and identical tree topology was observed using various different tree-making algorithms. Moreover, all but one strain, S. flocculus NRRL B-2465T, exhibited identical gene sequences for all of the 5 house-keeping gene loci sequenced, but NRRL B-2465T exhibited a MLSA evolutionary distance of 0.005 from the other strains which is lower than the 0.007 MLSA evolutionary distance threshold proposed for species-level relatedness. These data support a proposal reclassify S. almquistii, S. flocculus, S. gibsonii, and S. rangoonensis as later heterotypic synonyms of Streptomyces albus with NRRL B-1811T as the type strain. The MLSA sequence data also demonstrated utility for quickly and conclusively confirming that numerous strains within the ARS Culture Collection were previously misidentified as subspecies of Streptomyces albus.

Last Modified: 9/10/2014
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