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United States Department of Agriculture

Agricultural Research Service

Research Project: Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry

Location: Endemic Poultry Viral Diseases Research Unit

Title: NDV HN gene C-terminal extension is not the determinant of the enteric tropism but influences the virus virulence

Authors
item Zhao, Wei -
item Yu, Qingzhong
item Zsak, Laszlo

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: January 18, 2013
Publication Date: July 28, 2013
Citation: Zhao, W., Yu, Q., Zsak, L. 2013. NDV HN gene C-terminal extension is not the determinant of the enteric tropism but influences the virus virulence. In: Proceedings of the 2013 annual meeting of the American Association of Avian Pathologists, July 20-23, 2013, Chicago, Illinois. CDROM, p.80.

Technical Abstract: Many asymptomatic enteric Newcastle disease virus (NDV) strains contain a larger hemagglutinin-neuraminidase (HN) protein (616 amino acids, aa) than that (571 aa) of virulent respirotropic NDV strains. Therefore, it has been suspected that the 45 aa extension at the C-terminus of HN influences the virus virulence and tissue tropism. In the present study, we generated two NDV respirotropic strain-based recombinant viruses with the 45 aa extension at its HN protein C-terminus using reverse genetics technology. The biological analysis of these recombinant viruses has demonstrated that the HN protein C-terminal extension is not the determinant of the NDV enteric tropism, but decreases virus virulence.

Last Modified: 9/1/2014
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