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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC RESEARCH TO ENHANCE EFFICIENT AND SUSTAINABLE PRODUCTION OF BEEF CATTLE AND SHEEP

Location: Genetics, Breeding, & Animal Health

Title: Estimation of genetic marker effects for CAPN1, CAST, and GHR on carcass quality traits in Angus cattle selected to increase minor marker frequencies

Authors
item Tait Jr, Richard
item Shackelford, Steven
item Wheeler, Tommy
item King, David
item Casas, Eduardo
item Thallman, Richard
item Smith, Timothy
item Bennett, Gary

Submitted to: Midwestern Section of the American Society of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: November 28, 2012
Publication Date: March 1, 2013
Citation: Tait Jr, R.G., Shackelford, S.D., Wheeler, T.L., King, D.A., Casas, E., Thallman, R.M., Smith, T.P., Bennett, G.L. 2013. Estimation of genetic marker effects for CAPN1, CAST, and GHR on carcass quality traits in Angus cattle selected to increase minor marker frequencies [abstract]. Journal of Animal Science. 91(Supplement 2):62-63. Abstract No. O186.

Technical Abstract: Genetic marker effects and interactions cannot be accurately estimated when minor marker allele frequencies (MAF) are low. To increase the accuracy of estimation for three marker systems in commercial use, an Angus population at USMARC was subjected to marker assisted-selection for multiple years to increase MAF or frequencies of divergent haplotypes (FDH). Substantial increases in FDH and MAF were achieved, with SNP haplotypes in the µ-calpain 1 gene (CAPN1) (haplotypes C-C or G-T at markers CAPN1_316 and CAPN1_4751, respectively) with haplotype increases from 0.456 to 0.530 and 0.242 to 0.363, respectively, and individual markers in the calpastatin (CAST) and growth hormone receptor (GHR) genes with increases from 0.080 to 0.348, and 0.182 to 0.421, respectively. 199 steers born between spring of 2009 and 2011 from this population (24 sires and 155 dams) were evaluated for: final live weight, dressing percent, hot carcass weight, carcass traits measured by the VBG2000 grading camera {adjusted 12th rib fat thickness, 12th rib longissimus area, marbling, calculated vision yield grade (VYG)}, slice shear force (SSF), and SSF predicted by visible and near-infrared reflectance spectroscopy. Statistical model included effects of marker (CAPN1, CAST, and GHR), year of birth, age of calf, age of dam, and additive polygenic relationships based on a pedigree of 7,433 animals. CAST had a significant effect (P<0.05) on VYG with no evidence (P>0.10) of dominance inheritance. Significant effects were identified for both CAPN1 and CAST (P<0.001 for both) on SSF. There was no evidence (P>0.10) of dominance inheritance for either CAPN1 or CAST on SSF. Furthermore, there was no evidence of an interaction (P>0.10) between CAPN1 and CAST on SSF. Difference between homozygous for the tender and tough genotypes at both CAPN1 and CAST was -4.2 kg (35% of the mean) for SSF. All other genetic marker effects were not significant (P>0.10) for other traits evaluated in this study. These results provide a better understanding of marker effects, mode of inheritance, and independence of markers on beef quality traits.

Last Modified: 10/21/2014