ARS | Publication request: Optimizatin of sugarcane factory application of Commercial Dextranases in the U.S.

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: April 5, 2004
Publication Date: April 7, 2004
Citation: Eggleston, G., Monge, A. 2004. Optimization of sugarcane factory application of commercial dextranases in the U.S. In: Proceedings of the 2004 Sugar Processing Research Conference, April 4-7, 2004, Atlanta, Georgia. p. 371-394.

Technical Abstract: Although the use of commercial dextranases to break down dextran in sugar manufacture was pioneered by Australian researchers in the 1970s, applications in the U.S. sugar industry are still not optimized. This is partly because of misinformation about where to add the enzyme and which enzyme to use. Furthermore, there is no national or international method to measure the activity of dextranases, which has meant that direct comparison of activities is not possible. In this dextranase optimization study, a simple method to determine the relative activity of dextranases was identified, which is based on titration and could be easily undertaken at factories. All the relative activities were further confirmed with ion chromatography with integrated pulsed amperometry detection (IC-IPAD) using a sodium hydroxide/sodium acetate gradient method.