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United States Department of Agriculture

Agricultural Research Service

Research Project: Primary and Secondary Prevention of Peanut and Tree Nut Allergy

Location: Food Processing and Sensory Quality Research

Title: Effects of reduction and proteolysis on cashew allergens

Authors
item Mattison, Chris
item Desormeaux, Wendy
item Grimm, Casey

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: December 20, 2012
Publication Date: N/A

Technical Abstract: Allergic reaction to cashew ingestion is frequently more severe than reaction to peanut ingestion, and food allergens are commonly resistant to digestive proteases. The purpose of this study was to characterize the sensitivity of cashew proteins to proteolysis. Cashew protein extracts and purified cashew allergens were subjected to proteolysis with varying protease-protein ratios and conditions. The effects of proteolysis were visualized by SDS-PAGE and immunoassay. Proteins and peptides were also evaluated by HPLC-coupled mass-spectrometry, and spectral data from the analysis were evaluated with Spectrum Mill software to further compare and identify protein fragments. Protease concentration and prior treatment with reducing agents influenced the susceptibility of cashew proteins to proteolysis. Our results indicate that processing steps including reduction and proteolysis could be used to reduce cashew allergen levels.

Last Modified: 7/23/2014
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