Title: Reverse genetics of avian metapneumoviruses Author
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2012
Publication Date: N/A
Technical Abstract: An overview of avian metapneumovirus (aMPV) infection in turkeys and development of a reverse genetics system for aMPV subgroup C (aMPV-C) virus will be presented. By using reverse genetics technology, we generated recombinant aMPV-C viruses containing a different length of glycoprotein (G) gene or a deletion of the M2-2 gene. The biological properties of these recombinant viruses were assessed in Vero cells and in specific-pathogen-free turkeys. The results demonstrated that the large portion of the extracellular domain of the viral attachment protein and the M2-2 gene are not essential for virus viability in cell cultures and in turkeys, but may be required for sufficient virus replication to maintain virus pathogenicity and immunogenicity in a natural host.