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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION OF DISEASE MECHANISMS AND CONTROL STRATEGIES FOR BACTERIAL RESPIRATORY PATHOGENS IN CATTLE Title: Development of a PCR assay for identification of Bordetella hinzii

Author
item REGISTER, KAREN

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 8, 2013
Publication Date: June 1, 2013
Citation: Register, K.B. 2013. Development of a PCR assay for identification of Bordetella hinzii. Avian Diseases. 57(2):307-310.

Interpretive Summary: The bacterium Bordetella hinzii infects primarily poultry and immunocompromised humans. Although initially thought to be nonpathogenic in poultry, it was recently shown that some strains cause disease in turkey poults indistinguishable from the clinical presentation of turkey coryza caused by the closely related bacterium Bordetella avium. Because B. hinzii and B. avium share many characteristics, distinguishing between these bacteria is difficult and there is currently no method for identification of B. hinzii suitable for use by diagnostic laboratories. This report details a rapid and highly accurate PCR method for identification of B. hinzii that will be of value in the diagnosis of both veterinary and human infections.

Technical Abstract: Bordetella hinzii infects primarily poultry and immunocompromised humans. Although initially thought to be nonpathogenic in poultry, it was recently shown that some strains cause disease in turkey poults indistinguishable from the clinical presentation of turkey coryza caused by Bordetella avium. B. hinzii and B. avium are closely related and share many genetic and phenotypic traits. Distinguishing between these bacteria is difficult and there is no method for identification of B. hinzii suitable for use by diagnostic laboratories. The ompA gene was identified as a potential target for a B. hinzii-specific PCR based on DNA sequence comparisons of amplicons from several candidate genes obtained from a limited number of B. hinzii and B. avium isolates. A PCR assay based on the ompA gene was designed for further testing. Assay sensitivity is 100% based on analysis of 48 B. hinzii isolates from diverse geographic locations representing all known ribotypes. Evaluation of 71 isolates of B. avium and 20 other bacterial isolates from poultry, comprising gram-negative and gram-positive commensals and pathogens of 9 genera, demonstrated an assay specificity of 100%. Reproducibility is 100% using either purified genomic DNA or cell lysates. The ompA PCR is a rapid, reliable and accurate method for identification of B. hinzii. It provides a valuable new tool for veterinary diagnostic laboratories investigating poultry respiratory disease outbreaks and may also be of value in the diagnosis of human infection.

Last Modified: 9/10/2014
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