Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Proceedings
Publication Acceptance Date: September 14, 2012
Publication Date: December 21, 2012
Citation: Park, W., Bauer, P.J., Scheffler, B.E., Bowman, M.J., Campbell, B.T. 2012. Sub-genomic level sequence analysis of the aquaporin multi-gene family in cotton [Abstract]. In: Proceedings of the International Cotton Genome Initiative 2012 Conference, October 8-12, Raleigh, North Carolina. 50. 2012 CDROM. Technical Abstract: Aquaporins function mainly as water transport channel proteins that facilitate water movement across intracellular and intercellular membranes in most living organisms. Plant aquaporins belong to a multi-gene family and are commonly categorized into 5 subfamilies according to sequence similarity. Recently, we identified large numbers of aquaporin genes using cloning strategies and homology searches in upland cotton (Gossypium hirsutum L.). This finding prompted us to investigate the sub-genomic origin of aquaporins in conjunction with the effort to identify allele-specific single nucleotide polymorphism markers among highly homeologous aquaporin genes in the complicated upland cotton genome. For this purpose, we are using a number of approaches to compare the sequence of aquaporin genes in genomes representing cultivated tetraploid cotton species and their ancestral diploid species. Based on our analysis of the draft D-genome sequence, our results show that several close members of aquaporins are clustered in specific chromosomes; this genomic feature is conserved among genomes of higher plants. Understanding the structural genomic organization of aquaporin genes in tetraploid cotton will facilitate efforts to deploy them in cotton breeding programs.