Technical Abstract: Cleaning and sanitation in food processing facilities is a critical step in reducing the risk of transfer of pathogenic organisms to food consumed by the public. Current methods to check the effectiveness of sanitation procedures rely on visual observation and sub-sampling tests such as ATP bioluminescence assays and culturing methods. To augment existing verification methods, a hand-held VIS hyperspectral imaging device was developed. The device is capable of acquiring reflectance images using ambient lighting, and fluorescence responses to supplemental violet (405 nm) excitation. To enhance the ability of detecting relatively low intensity fluorescence responses in the presence of ambient lighting, the device includes the ability to set and display camera gain by wavelength. This ability allows identification of response regions where ambient illumination intensity is low, commonly around 475, 520, and 675 nm. A principle goal is to acquire data to support development of a commercially-viable, hand-held, imaging system.