Technical Abstract: Citrus huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide. The causal agents of HLB are three species of a-proteobacteria, ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. Liberibacter africanus’ and ‘Ca. Liberibacter americanus’. Previous studies have found distinct variations in temperature sensitivity and tolerance among these species. Here we describe the use of controlled heat treatments to cure HLB caused by Las, the most prevalent and heat tolerant species. Using temperature-controlled growth chambers, we evaluated the time duration and temperature required to suppress or eliminate the Las bacterium in citrus, using various temperature treatments for time periods ranging from 2 days to 4 months. Results of quantitative polymerase chain reaction (qPCR) after treatment illustrate significant decreases in the Las bacterial titer, combined with healthy vigorous growth by all surviving trees. Repeated qPCR testing confirmed that previously infected, heat treated plants showed no detectable levels of Las, while untreated control plants remained highly infected. Continuous thermal exposure to 40-42°C for a minimum of 48 hours was sufficient to significantly reduce titer or eliminate Las bacteria entirely in HLB-affected citrus seedlings. This method may be useful for the control of Liberibacter-infected plants in nursery and greenhouse settings.