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United States Department of Agriculture

Agricultural Research Service

Research Project: PRESERVATION AND QUALITY ASSESSMENT OF PLANT GENETIC RESOURCES Title: High-throughput genotyping of Lesquerella (physaria and paysonia) Germplasm collections using DArT markers

Authors
item Cruz, Von Mark
item Kilian, Andrzej -
item Dierig, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 12, 2012
Publication Date: November 12, 2012
Citation: Cruz, V.V., Kilian, A., Dierig, D.A. 2012. High-throughput genotyping of Lesquerella (physaria and paysonia) Germplasm collections using DArT markers. Meeting Abstract. AAIC annual meeting Sonoma CA, November 12-15, 2012.

Technical Abstract: The Physaria (syn. Lesquerella) and Paysonia germplasm collection of the U.S. National Plant Germplasm System has not been fully characterized for genetic diversity. There are currently more than 200 accessions being managed ex situ to support new crop research and development activities. To better understand the extent of diversity in the collection of Physaria and Paysonia, DArT (Diversity Array Technology) marker platforms were developed and utilized to characterize the P. fendleri collection together with other representative species. A total of 86 accessions from 12 species were used to develop two types of marker platforms. The first resulted in 2,833 polymorphic markers, and the second, 27,748 high quality markers, both have a scoring reproducibility greater than 99%. Cluster analysis successfully classified the representative species by geographical source and breeding status. Previous taxonomic group classification was also found concordant with results of molecular marker analysis. The analysis of genetic structure revealed significant genetic differentiation among the assigned population groups in P. fendleri. The additional information provided by molecular characterization of the Physaria and Paysonia collections will make possible more efficient management of the germplasm. The DArT platforms will also be useful in future molecular breeding work in P. fendleri and other species.

Last Modified: 12/20/2014
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