Submitted to: Slovenian Genetics Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 18, 2012
Publication Date: September 27, 2012
Citation: Duangjit, J., Bonanec, B., Chan, A., Town, C., Havey, M.J. 2012. Transcriptome sequencing to produce a SNP-based genetic map of onion. Slovenian Genetics Society Meeting. p. 45.
Sequencing the onion genome is challenging because of its enormous size (16 giga base pairs DNA per haploid genome). Pilot sequencing of onion transcripts showed sufficient numbers of single nucleotide polymorphisms (SNPs) to develop a detailed genetic map. We sequenced 2.5 Roche-454 plates of normalized cDNAs from two parental lines of onion: 5225B is a red, doubled-haploid onion and OH-1 is a yellow inbred that showed a high frequency of gynogenic haploid production. Over 1200 SNPs between the two parental lines were randomly chosen for genetic mapping. Gynogenic haploids were extracted from red hybrids from the cross of OH-1 with 5225B. Individual haploid plants were asexually propagated and evaluated in replicated trials for bulb quality traits without allelic interactions.