Location: Plant Germplasm Introduction and Testing
Title: Mycobiota of Lupinus albus seed from a public germplasm collection Authors
Submitted to: North American Fungi
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 24, 2013
Publication Date: January 24, 2013
Citation: Alomran, M.M., Lupien, S.L., Coyne, C.J., Dugan, F.M. 2013. Mycobiota of Lupinus albus seed from a public germplasm collection. North American Fungi. 8(4):1-15. Interpretive Summary: It is important to assess the seed health of germplasm distributed from public germplasm accessions. Representative samples of 14 accessions from the USDA-ARS Western Regional Plant Introduction Station (a unit of the National Plant Germplasm System) were subjected to standard protocols to assay for seedborne fungi. Fungi were identified by both morpho-cultural characters and DNA sequence analyses. Although species belonging to 10 genera were recovered and preserved, none belonged to species known to be major pathogens of lupine. Approximately 10% of seeds assayed contained internal fungi. Most were saprophytes or fungi that can cause damping off of seedlings under conditions adverse to seedling health. One minor pathogen of legumes, Fusarium acuminatum, was detected. Total Fusarium species accounted for ~3% of the fungi recovered and preserved.
Technical Abstract: Seedborne mycobiota of Lupinus albus was assessed using blotter paper and agar media with Rose Bengal or semi-selective for Pythium or Fusarium. Samples of 200 seeds were taken from each of 16 inventories, comprising 14 accessions originating from Germany, France, Ukraine, Syria, Hungary or Spain, and increased 1-2 times by the USDA-ARS Western Regional Plant Introduction Station. A total of 307 fungal isolates were recovered and preserved with over 96% belonging to 10 genera: Penicillium (47%), Cladosporium (32%, including Cladosporium-like Chalastospora), Alternaria (4%), Aspergillus (4%), Fusarium (3%), Rhizopus (3%), Ulocladium (3%), Chaetomium (0.5%) and Arthrinium (< 0.5%). By sub-sampling, fungi were further identified by morpho-cultural characters and/or DNA sequences as Alternaria infectoria, A. alternata, and A. tenuissima species-groups, Arthrinium arundinus, Aspergillus niger, Chalastospora gossypii, Cladosporium herbarum and C. cladosporioides species complexes, Chaetomium sp., Fusarium acuminatum, Rhizopus stolonifer, and Ulocladium atrum group. Penicillia included P. hordei, but most penicillia could not be assigned species names either by morphology or ß-tubulin DNA sequence.