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United States Department of Agriculture

Agricultural Research Service

Research Project: Biting and Stinging Pests: Ecology and Biologically-base Control

Location: Biological Control of Pests Research Unit

Title: Inhibitory activities of venom alkaloids from the red imported fire ant, Solenopsis invicta Buren, against the growth of Clavibacter michiganensis subsp. michiganensis (E.F. Smith) Davis et al. in vitro and the application of

Authors
item Li, Shezeng -
item Jin, Xixuan
item CHEN, JIAN
item Lu, Shien -

Submitted to: International Journal of Pest Management
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 9, 2013
Publication Date: April 16, 2013
Citation: Li, S., Jin, X., Chen, J., Lu, S. 2013. Inhibitory activities of venom alkaloids from the red imported fire ant, Solenopsis invicta Buren, against the growth of Clavibacter michiganensis subsp. michiganensis (E.F. Smith) Davis et al. in vitro and the application of. International Journal of Pest Management. 59:150-156.

Interpretive Summary: Bacterial canker of tomato is a highly destructive disease and has caused major economic losses in tomato production worldwide. This report describes our findings of using piperidine and piperideine alkaloids extracted from the venom of red imported fire ant against the growth of this destructive plant pathogenic bacterium, Clavibacter michiganensis subsp. michiganensis (CMM) in laboratory and the application of piperidine alkaloids to manage the symptom development of CMM canker on tomato in the greenhouse. Both piperidine and piperideine alkaloids significantly inhibited CMM growth on nutrient agar plates. The inhibitory activity of piperidine alkaloids was stable during storage at room and high temperatures. Piperidine alkaloids also significantly reduced the symptom development expressed as lesion numbers on two tomato cultivars, Better Boy and DRK7018F1 in the greenhouse. Our discovery may lead to the development of new types of safer fungicides.

Technical Abstract: Bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis (CMM) is a highly destructive disease and has caused major economic losses in tomato production worldwide. There are limited methods available to manage this disease. In searching for disease management alternatives, the inhibitory activity of alkaloids extracted from the red imported fire ant was studied in the laboratory and greenhouse. Both piperidine and piperideine alkaloids significantly inhibited CMM growth on nutrient agar plates. The inhibitory activity of piperidine alkaloids was stable at 4oC and 22oC for 12 weeks and at 54oC for 4 weeks. The growth of CMM was negatively correlated with the concentration of piperidine alkaloids in nutrient broth (R2 = 0.948). Piperidine alkaloids also significantly reduced the symptom development expressed as lesion numbers on two tomato cultivars, Better Boy and DRK7018F1 in the greenhouse. This is the first report that demonstrates both piperidine and piperideine alkaloids extracted from the red imported fire ant are highly inhibitory against a plant pathogenic bacterium CMM and that piperidine alkaloids can provide satisfactory management of CMM bacterial canker on tomato seedlings in the greenhouse. These findings may lead to the development of a new group of bactericides.

Last Modified: 7/28/2014