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United States Department of Agriculture

Agricultural Research Service

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Laboratory

Title: Analysis of genetic diversity within the Puccinia graminis Ug99 race group using SNP based markers

Author
item Szabo, Les

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2012
Publication Date: August 28, 2012
Citation: Szabo, L.J. 2012. Analysis of genetic diversity within the Puccinia graminis Ug99 race group using SNP based markers. Meeting Abstract. p. 237.

Technical Abstract: Since the first discovery of Ug99 (race TTKSK) six additional race variants of Puccinia graminis f. sp. tritici (Pgt) have been found. Standard race typing using wheat differential lines provides very useful phenotypic information, but little is known about the underlying genetic variation within this race group. DNA sequence data from nine isolates of Pgt were mined to identify SNPs that were polymorphic between members of the Ug99 race group. Thirty-four SNP markers were developed and used to analyze the genetic diversity of 106 Pgt isolates from the Ug99 race group. This collection of isolates represented six of the seven known members of the Ug99 race group: TTKSK, 42 isolates; TTKST, 19 isolates; TTTSK, 15 isolates; TTKSF, 19 isolates; TTKSP, 5 isolates; and PTKSP, 6 isolates. Nineteen different genotypes were observed and the number of genotypes per race phenotype ranged from 1 to 6 with TTKSK containing the most genotypes. Principle coordinate analysis showed that three of the race phenotypes (TTKSF, TTKSK and TTTSK) each contained two distinctive genetic groups suggesting that specific race phenotypes have likely evolved multiple times from different genetic backgrounds.

Last Modified: 7/30/2014
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