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Agricultural Research Service United States Department of Agriculture
 
Research Project: INTERVENTIONS TO REDUCE FOODBORNE PATHOGENS IN SWINE AND CATTLE

Location: Food and Feed Safety Research

Title: Rapid detection of Salmonella in bovine lymph nodes using a commercial real-time PCR system

Authors
item Hill, Joshua
item Edrington, Thomas
item Loneragan, Guy -
item Gragg, Sarah -
item Nisbet, David

Submitted to: Journal of Food Protection
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 8, 2013
Publication Date: N/A

Interpretive Summary: Cattle may contain the bacteria Salmonella that can make people sick. Food production facilities routinely test their products for contamination. This report describes development of a rapid method for testing for the bacteria Salmonella. This rapid testing may allow food production facilities a quicker turn around to test their perishable products.

Technical Abstract: Rapid Salmonella detection is needed to help prevent the distribution of contaminated food products. Using traditional culture methods, Salmonella detection can take up to 3-5 days. Using an improved protocol and a commercial real-time PCR system, we have shortened the detection time to under 24 hours with the same sensitivity as compared to traditional Salmonella culture methods.

     
Last Modified: 05/21/2013