ARS | Publication request: Rapid detection of Salmonella in bovine lymph nodes using a commercial real-time PCR system

Submitted to: PostDoc Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 8, 2013
Publication Date: March 1, 2013
Citation: Hill, J.E., Edrington, T.S., Loneragan, G., Gragg, S.E., Nisbet, D.J. 2013. Rapid detection of Salmonella in bovine lymph nodes using a commercial real-time PCR system. PostDoc Journal. 1:35-39.

Interpretive Summary: Cattle may contain the bacteria Salmonella that can make people sick. Food production facilities routinely test their products for contamination. This report describes development of a rapid method for testing for the bacteria Salmonella. This rapid testing may allow food production facilities a quicker turn around to test their perishable products.

Technical Abstract: Rapid Salmonella detection is needed to help prevent the distribution of contaminated food products. Using traditional culture methods, Salmonella detection can take up to 3-5 days. Using an improved protocol and a commercial real-time PCR system, we have shortened the detection time to under 24 hours with the same sensitivity as compared to traditional Salmonella culture methods.