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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #283086
Title: A public pathogen collection for characterizing disease resistance in alfalfa cultivars

Author
item Samac, Deborah - Debby
item Dornbusch, Melinda - Mindy
item MCLAUGHLIN, DAVID - University Of Minnesota

Submitted to: North American Alfalfa Improvement Conference
Publication Type: Abstract Only
Publication Acceptance Date: 6/27/2012
Publication Date: 7/10/2012
Citation: Samac, D.A., Dornbusch, M.R., Mclaughlin, D.J. 2012. A public pathogen collection for characterizing disease resistance in alfalfa cultivars [abstract]. 2012 Joint North American Alfalfa Improvement Conference, Trifolium, & Grass Breeders Conference, July 10-12, 2012, Ithaca, New York. Available: http://www.naaic.org/Meetings/National/2012meeting/T21-Samac.pdf.

Interpretive Summary:

Technical Abstract: Standard tests have been developed by the North American Alfalfa Improvement Conference to characterize disease resistance in alfalfa cultivars. To support these tests, a collection of pathogens has been made as part of the University of Minnesota Mycological Collection and is available to the alfalfa research community. In preparation for depositing into the collection, each strain was verified to be a pure culture and identified by sequencing of rDNA. Mycelial plugs from fresh cultures were removed from culture plates, placed in cryovials with a cryoprotectant, cooled slowly (-1 degree/sec) to -70 degrees C, and then transferred to liquid nitrogen cryogenic storage. Viability of cultures was tested by plating representative samples. Cultures are available upon request. It is the responsibility of the requester to obtain the necessary permits for transfer of cultures.