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United States Department of Agriculture

Agricultural Research Service

Research Project: BIOLOGY, EPIDEMIOLOGY, PATHOGENESIS, AND VECTOR SPECIFICITY OF SUGARBEET AND VEGETABLE VIRUSES

Location: Crop Improvement and Protection Research

Title: Complete genome sequence and biological characterization of Moroccan pepper virus (MPV) and reclassification of Lettuce necrotic stunt virus as MPV

Authors
item Wintermantel, William
item Hladky, Laura

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 19, 2012
Publication Date: May 1, 2013
Citation: Wintermantel, W.M., Hladky, L.L. 2013. Complete genome sequence and biological characterization of Moroccan pepper virus (MPV) and reclassification of Lettuce necrotic stunt virus as MPV. Phytopathology. 105:501-508.

Interpretive Summary: Moroccan pepper virus (MPV) and Lettuce necrotic stunt virus (LNSV) have been steadily increasing in prevalence in central Asia and western North America, respectively over the past decade. Recent sequence analysis of LNSV demonstrated a close relationship between the coat proteins of LNSV and MPV. To determine the full extent of the relationship between LNSV and MPV, the genomes of three MPV isolates were sequenced and compared with that of LNSV. Sequence analysis demonstrated that genomic nucleotide sequences as well as virus encoded proteins of the three MPV isolates and LNSV shared 97% or greater identity. A full-length infectious clone of LNSV was developed and viral RNA produced from this clone induces symptoms identical to those of the original isolate on a series of host plants. These unique host reactions are similar to those described previously for MPV. Collectively these molecular and biological results demonstrate that LNSV should be classified as MPV within the family Tombusviridae, genus Tombusvirus.

Technical Abstract: Moroccan pepper virus (MPV) and Lettuce necrotic stunt virus (LNSV) have been steadily increasing in prevalence in central Asia and western North America, respectively over the past decade. Recent sequence analysis of LNSV demonstrated a close relationship between the coat proteins of LNSV and MPV. To determine the full extent of the relationship between LNSV and MPV, the genomes of three MPV isolates were sequenced and compared with that of LNSV. Sequence analysis demonstrated that genomic nucleotide sequences as well as virus encoded proteins of the three MPV isolates and LNSV shared 97% or greater identity. A full-length clone of a California LNSV isolate was developed and infectious transcripts induced symptoms identical to those of the original isolate on a series of host plants. These unique host reactions are similar to those described previously for MPV. Collectively these molecular and biological results demonstrate that LNSV should be classified as MPV within the family Tombusviridae, genus Tombusvirus.

Last Modified: 4/25/2014
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