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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Cryopreservation and maintenance of hop material in the USDA germplasm collection

Authors
item Reed, Barbara
item Hummer, Kim

Submitted to: Acta Horticulture Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 13, 2012
Publication Date: September 20, 2012
Citation: Reed, B.M., Hummer, K.E. 2012. Cryopreservation and maintenance of hop material in the USDA germplasm collection. Acta Horticulture Proceedings. p.63.

Interpretive Summary: The US Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR) has the responsibility for conservation of the hop (Humulus L.) genetic resources. The collection includes about 530 accessions representing 7 species from 21 countries. This germplasm includes wild collected seeds, virus and viroid tested plants growing in greenhouses or screenhouses, in vitro cultures, and cryogenically preserved pollen and meristems. The tissue culture collection is primarily composed of pathogen-tested cultivars and wild collected species. The cryopreserved clonal collection was established from the tissue cultured plants and includes 73 accessions. These cryopreserved samples are safeguarded at the USDA ARS National Center for Genetic Resources Preservation in Ft. Collins, Colorado. Hop stunt viroid recently appeared in the main hop production areas of the Pacific Northwestern United States. The plants NCGR distributes have tested negative for this viroid. Hop germplasm from the NCGR is distributed to researchers internationally in accordance with country, regional and state phytosanitary regulations.

Technical Abstract: The US Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR) has the responsibility for conservation of the hop (Humulus L.) genetic resources. The collection includes about 530 accessions representing 7 taxa (species and subspecies) from 21 countries. This germplasm includes wild collected seeds, virus and viroid tested plants growing in greenhouses or screenhouses, in vitro cultures, and cryogenically preserved pollen and meristems. The tissue culture collection is primarily composed of pathogen-tested cultivars and wild collected species. The cryopreserved clonal collection was established from the tissue cultured plants and includes 73 accessions stored using the encapsulation-dehydration technique. These cryopreserved samples are safeguarded at the USDA ARS National Center for Genetic Resources Preservation in Ft. Collins, Colorado. Hop stunt viroid recently appeared in the main hop production areas of the Pacific Northwestern United States. The plants NCGR distributes have tested negative for this viroid. Hop germplasm from the NCGR is distributed to researchers internationally in accordance with country, regional and state phytosanitary regulations.

Last Modified: 8/22/2014
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