Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 15, 2012
Publication Date: July 10, 2012
Citation: Feng, S., Chen, X., Pandey, M., Liu, Z., Culbreath, A., Guo, B., Holbrook Jr, C.C. 2012. Two putative TSWV resistance gene-specific markers linked to genetic linkage group in peanut [abstract]. American Peanut Research and Education Society Abstracts. Technical Abstract: Among the diseases in peanut, tomato spotted wilt virus (TSWV) is the most destructive viral disease threatening peanut production in the Southeastern U.S. Host-plant resistance through use of resistant cultivars is the best approach compared to other available strategies for controlling this devastating disease. In this study, through data mining two peanut EST sequences with high similarity to tomato Sw-5 were identified for further investigation. Two putative genes were obtained using 5’ and 3’-RACE, named Ahsw-1 and Ahsw-2, respectively. Two gene-specific marker(s) were developed from these two putative TSWV resistance genes and used to amplify four parental lines of two mapping populations segregating for this disease for polymorphism. Genotypic data were generated on one of these mapping population possessing 353 recombinant inbred lines (RILs) derived from SunOleic 97R and NC94022. The two markers, Ahsw-1and Ahsw-2 were mapped to linkage group 4 of the S population between SSR markers Seq2F10-110 and TC7G10-200 along with 6 other markers. Further study will be continued to study association of these two markers with TSWV resistance.