ARS | Publication request: UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 2012
Publication Date: April 21, 2012
Citation: Hughes, S.R., Rich, J.O. 2012. UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars [abstract]. American Chemical Society.

Technical Abstract: More robust industrial yeast strains from Kluyveromyces marxianus NRRL Y-1109 and have been produced using UV-C irradiation specifically for anaerobic conversion of lignocellulosic sugar streams to fuel ethanol at elevated temperature (45°C). This type of random mutagenesis offers the possibility of generating large numbers of mutations broadly over the whole genome. When all of the genes were mutagenized to average 80% kill level and selected in sufficient numbers using automated plating strategies, we observed strains with improved utilization of sugars from lignocellulosic feedstocks under anaerobic conditions and at elevated temperatures.