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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Uv-C Mutagenesis of Kluyveromyces Marxianus Nrrl Y-1109 Strain for Improved Anaerobic Growth at Elevated Temperature on Pentose and Hexose Sugars

Authors
item Hughes, Stephen
item Rich, Joseph

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 2012
Publication Date: April 21, 2012
Citation: Hughes, S.R., Rich, J.O. 2012. UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars [abstract]. American Chemical Society.

Technical Abstract: More robust industrial yeast strains from Kluyveromyces marxianus NRRL Y-1109 and have been produced using UV-C irradiation specifically for anaerobic conversion of lignocellulosic sugar streams to fuel ethanol at elevated temperature (45°C). This type of random mutagenesis offers the possibility of generating large numbers of mutations broadly over the whole genome. When all of the genes were mutagenized to average 80% kill level and selected in sufficient numbers using automated plating strategies, we observed strains with improved utilization of sugars from lignocellulosic feedstocks under anaerobic conditions and at elevated temperatures.

Last Modified: 7/31/2014
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