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United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTIONS TO REDUCE FOODBORNE PATHOGENS IN SWINE AND CATTLE

Location: Food and Feed Safety Research

Title: Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest

Authors
item Gragg, Sara -
item Loneragan, Guy -
item Brashears, Mindy -
item Arthur, Terrance
item Bosilevac, Joseph
item Kalchayanand, Norasak
item Wang, Rong
item Schmidt, John
item Brooks, James
item Shackelford, Steven
item Wheeler, Tommy
item Brown, Tyson -
item Edrington, Thomas
item Harhay, Dayna

Submitted to: Foodborne Pathogens and Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 8, 2013
Publication Date: April 8, 2013
Repository URL: http://handle.nal.usda.gov/10113/56897
Citation: Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.

Technical Abstract: Bovine peripheral lymph nodes, including subiliac lymph nodes, have been identified as a potential source of human exposure to Salmonella enterica when trim containing these nodes is incorporated into ground beef. In order to gain a better understanding of the burden of S. enterica in subiliac lymph nodes of feedlot and cull cattle at harvest, 3,327 subiliac lymph nodes were collected from cattle in 3 geographically distinct regions of the U.S. Overall crude prevalence of S. enterica was 8.0% (95% confidence limits [CL]=7.1, 8.9%). Prevalence was greater in feedlot cattle (15.5% [95% CL=13.7, 17.4%]) compared to cull cattle (1.8% [95% CL= 1.2, 2.4%]). Of the 618 feedlot cattle lymph nodes subjected to enumeration, 144 nodes harbored S. enterica, and estimates of concentration were <1.3 Log10 CFU/lymph node in 21.5% of nodes, 1.3 to 2.9 Log10 CFU/lymph node in 44.4%, 3.0 to 4.5 Log10 CFU/lymph node in 20.8%, and >4.5 log10 CFU/lymph node in 13.2%. The majority of serotypes recovered from lymph nodes were S. Montevideo (44.0%) and S. Anatum (24.8%). The majority (86.6%) of isolates were pansusceptible to a panel of 15 antimicrobial agents; however, multi-drug resistant isolates (7.1%) were also occasionally observed. Data indicate that Salmonella is readily recovered from the subiliac lymph nodes of cattle, and additional research is necessary to mitigate potential risk to food safety.

Last Modified: 4/19/2014
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