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Title: Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease resistance genes in common bean cultivar Ouro Negro

Author
item GONCALVES-VIDIGAL, M - University Of Maringa
item CRUZ, A - University Of Maringa
item LACANALLO, G - University Of Maringa
item VIDIGAL FILHO, P - University Of Maringa
item SOUZA, L - University Of Maringa
item PACHECO, C - University Of Maringa
item MCCLEAN, P - North Dakota State University
item GEPTS, P - University Of California
item Pastor Corrales, Marcial - Talo

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/21/2013
Publication Date: 6/13/2013
Citation: Goncalves-Vidigal, M., Cruz, A., Lacanallo, G.F., Vidigal Filho, P., Souza, L.L., Pacheco, C., Mcclean, P., Gepts, P., Pastor Corrales, M.A. 2013. Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease resistance genes in common bean cultivar Ouro Negro . Theoretical and Applied Genetics. 126(9):2245-2255.

Interpretive Summary: Anthracnose and angular leaf spot are very destructive diseases of common bean. We report here a genetic solution for the simultaneous management of these two diseases. Our study revealed that two disease resistance genes, one for the control of anthracnose and the other for the control of angular leaf spot, occur together in a highly productive black-seeded common bean named Ouro Negro. These two genes are located very close to each other on chromosome 4 of the common bean. Because of their close proximity, the two genes are inherited together. We identified two molecular markers that are also located in close proximity of the two resistance genes. These markers can be used to identify common bean plants having the two resistance genes. The results of this study are very useful to plant breeders using marker-assisted selection to develop common bean cultivars with resistance to the anthracnose and angular leaf spot diseases. The molecular markers would significantly reduce the time and cost associated with the simultaneous transferring of two disease resistance genes into new common bean cultivars.

Technical Abstract: Anthracnose (ANT) and angular leaf spot (ALS) are devastating diseases of common bean. Ouro Negro is a highly productive Mesoamerican black-seeded common bean cultivar possessing the dominant Co-10 and Phg-ON genes that confer resistance to ANT and ALS, respectively. In this study we elucidate the inheritance of resistance in Ouro Negro to the ANT and ALS diseases using a F2 population from the cross Ouro Negro (resistant) × Rudá (susceptible). Co-segregation analysis revealed that a single dominant gene in Ouro Negro confers resistance to races 73 and 63-39 of the ANT and ALS pathogens, respectively. The Co-10 and Phg-ON genes co-segregated and were at a distance of 0.9 cM from each other on linkage group Pv04. The molecular marker g2303, previously mapped to LG Pv04, was linked with Co-10 and Phg-ON at 0.9 cM and 1.8 cM, respectively. Similarly, the SF10 marker was linked with these genes at 7.8 and 8.7 cM, respectively. Allele segregation analysis in the BAT 93 × Jalo EEP 558 recombinant population showed that g2303 and SF10 segregated in the expected 1:1 ratio. Due to their physical linkage in a cis configuration, Co-10 and Phg-ON are inherited together and can be monitored indirectly with g2303 and SF10. We propose that the ANT and ALS resistance genes in Ouro Negro should be named as locus Co-10/Phg-8. These results will be very useful to breeding programs wanting to develop bean cultivars with ANT and ALS resistance using marker-assisted selection. The g2303 and SF10 markers will reduce the time and costs of pyramiding the Co-10 and Phg-On genes in commercial common bean cultivars.