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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGIES FOR PRODUCING RENEWABLE CHEMICALS AND POLYMERS FROM CARBOHYDRATES DERIVED FROM AGRICULTURAL FEEDSTOCKS

Location: Renewable Product Technology Research Unit

Title: Ingestion of a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex affected growth performance and fermentative and immunological characteristics of broiler chicks challenged with Salmonella typhimurium

Authors
item Faber, T -
item Dilger, R -
item Iakiviak, M -
item Hopkins, A -
item Price, Neil
item Fahey, G -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 19, 2012
Publication Date: July 19, 2012
Citation: Faber, T.A., Dilger, R.N., Hopkins, A.C., Price, N.P., Fahey, G.C. 2012. Ingestion of a novel galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex affected growth performance and fermentative and immunological characteristics of broiler chicks challenged with Salmonella typhimurium [abstract]. American Society of Animal Sciences.

Technical Abstract: Fermentable carbohydrates may enhance the ability of the gastrointestinal tract to defend against a pathogenic infection. We hypothesized that a galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex would positively impact immune status and prevent colonization and shedding in Salmonella typhimurium (ST)-infected chicks. Using a completely randomized design, one day old commercial broiler chicks (n=240; 4 replications/treatment; 5 chicks/replication) were assigned to 1 of 6 dietary treatments differing in concentration of GGMO-AX (0, 1, 2, or 4%) or containing 2% Safmannan (Saf) or 2% short-chain fructooligosaccharides (scFOS). Cellulose was used to make diets iso-total dietary fiber (TDF). On d 10 post-hatch, an equal number of chicks on each diet were inoculated with either phosphate buffered saline (sham control) or ST (1x10**8 CFU). All birds were euthanized on d 10 post-inoculation (PI) for collection of intestinal contents and select tissues. Overall, body weight gain and feed intake of chicks was greater (P < 0.05) in infected chicks PI, except for weight gain on d 0-3 PI. Gain:feed was affected (P < 0.05) by diet, with Saf-fed chicks having the highest G:F. The GGMO-AX substrate demonstrated prebiotic-like effects as indicated by increased cecal short-chain fatty acid concentrations, decreased cecal pH, and increased populations of Lactobacillus spp. and Bifidobacteria spp. as dietary GGMO-AX concentration increased. Excreta ST populations on d 5 and 10 PI, and ileal and cecal ST populations, tended to be affected (P < 0.10) by diet. Messenger RNA expression of IFN-' in the cecal tonsils was the only cytokine independently affected by infection and diet (P < 0.01). Chicks fed 2 and 4% GGMO-AX had similar expressions of IFN-y and IL-1ß, regardless of infection, suggesting that ST virulence was suppressed. Dietary supplementation with GGMO-AX resulted in prebiotic-like effects, but did not limit ST intestinal colonization or shedding, but possibly decreased the virulence of ST within the digestive tract.

Last Modified: 10/30/2014
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